Figure 5.
γ-tubulin–disrupted embryos have enhanced Patronin intensities. (A) Still frames revealing upregulated Patronin:GFP throughout the GBE in γ-tubulin37C shRNA (γ-tub) embryos as compared to control embryos. (B) Centrosomal Patronin:GFP intensities in control and γ-tub embryos; n = 150 centrosomal regions from k = 3 embryos for each background. (C) Perinuclear Patronin:GFP intensities in control and γ-tub embryos; n = 75 perinuclear regions from k = 3 embryos for each background. (D) Orthogonal view of MT natural log intensity heatmap showing enhanced MT bundles in γ-tub embryos compared to control. (E) Cross-section of MTs (green) and nuclei (magenta) showing enhanced and persistent perinuclear MT in γ-tub embryos as compared to control. (F) Centrosomal MT intensities in control and γ-tubulin37C embryos; n = 200 and 150 centrosomal regions for control and γ-tub, respectively, k = 4 for control and k = 3 γ-tub embryos. (G) Perinuclear MT intensities showing enriched MT pools in γ-tub perinuclear regions as compared to controls; n = 45 perinuclear regions k = 3 embryos for each background. (H) Orthogonal views of MT and nuclei showing MT bundles still shift apically in γ-tub embryos (arrows). Fig. 2 C; Fig. 3 C; Fig. 5, A and H; and Fig. 7, C and F control images/plots reproduced for comparison purposes. Scale bar = 5 μm. All scatter plots show the mean ± SD. Statistical significance was calculated using the Mann–Whitney U-test. ****P < 0.0001.

γ-tubulin–disrupted embryos have enhanced Patronin intensities. (A) Still frames revealing upregulated Patronin:GFP throughout the GBE in γ-tubulin37C shRNA (γ-tub) embryos as compared to control embryos. (B) Centrosomal Patronin:GFP intensities in control and γ-tub embryos; n = 150 centrosomal regions from k = 3 embryos for each background. (C) Perinuclear Patronin:GFP intensities in control and γ-tub embryos; n = 75 perinuclear regions from k = 3 embryos for each background. (D) Orthogonal view of MT natural log intensity heatmap showing enhanced MT bundles in γ-tub embryos compared to control. (E) Cross-section of MTs (green) and nuclei (magenta) showing enhanced and persistent perinuclear MT in γ-tub embryos as compared to control. (F) Centrosomal MT intensities in control and γ-tubulin37C embryos; n = 200 and 150 centrosomal regions for control and γ-tub, respectively, k = 4 for control and k = 3 γ-tub embryos. (G) Perinuclear MT intensities showing enriched MT pools in γ-tub perinuclear regions as compared to controls; n = 45 perinuclear regions k = 3 embryos for each background. (H) Orthogonal views of MT and nuclei showing MT bundles still shift apically in γ-tub embryos (arrows). Fig. 2 C; Fig. 3 C; Fig. 5, A and H; and Fig. 7, C and F control images/plots reproduced for comparison purposes. Scale bar = 5 μm. All scatter plots show the mean ± SD. Statistical significance was calculated using the Mann–Whitney U-test. ****P < 0.0001.

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