Patronin and acetylated MT intensities in γ-tubulin37C–disrupted embryos. (A) Still images showing Patronin:GFP enrichment at centrosomes (Asl:mCh) in control and CLASP embryos. Arrowheads point to the centrosomes. (B) Intensity heatmap for Patronin:GFP shown in orthogonal view in control and CLASP embryos, highlighting centrosomal enrichment of Patronin in CLASP embryos (arrows). (C) Orthogonal projection of Patronin:GFP intensity heatmap showing enhanced perinuclear (white arrows) and centrosomal (arrowheads) Patronin pools in γ-tub embryos as compared to control embryos. Green arrows indicate cortical Patronin. (D) Quantification showing MT intensities in apical regions above nuclei in γ-tubulin-compromised embryos as compared to the control at 20 min into GBE; n = 200 regions for control and n = 284 regions for γ-tub from k = 3 embryos for each background. (E) Scatter plot comparing centrosomal MT intensities in γ-tubulin and Patronin-γ-tubulin co-depleted embryos; n = 150 centrosomal regions from k = 3 embryos for each background. (F) Orthogonal view of acetylated MT color-coded for the intensity level in control and γ-tub embryos, and quantification is shown in (F′); n = 70 regions from k = 7 embryos for each background. Scale bar = 5 μm. All scatter plots show the mean ± SD. Statistical significance was calculated using the Mann–Whitney U-test. ns, not significant.