Figure 4.
Quantitation of centrosomal MTs in CLASP and Patronin embryos reveals an antagonistic relationship between centrosomal and ncMT networks. (A) γ-tubulin:GFP intensities vary in control, Patronin, and CLASP embryos. (B) Quantification of γ-tubulin:GFP in control, Patronin, and CLASP embryos at 0 and 20 min GBE; n = 150 for control and Patronin, and 200 centrosomes for CLASP at 0 min and n = 88, 150 and 172 centrosomes for control, Patronin and CLASP, respectively, at 20 min; control and Patronin k = 3 and CLASP k = 4 embryos. (C) Still images showing the localization of Patronin:GFP in control and CLASP embryos at 0 and 20 min GBE, highlighting the absence of perinuclear Patronin in CLASP embryos. (D) Perinuclear Patronin:GFP intensities in control and CLASP embryos 5 min before GBE onset showing depleted perinuclear intensities in CLASP embryos; n = 75 perinuclear regions from k = 3 embryos for each background. (E) Still images showing Patronin:GFP enrichment at centrosomes (5 μm below cell apices) in CLASP embryos unlike in control embryos (same control as Fig. 5 A and Fig. 7 A, 0 min at 5 μm), arrows mark centrosomal Patronin. (F) Centrosomal Patronin:GFP intensities at 0 and 20 min show upregulated centrosomal Patronin in CLASP compared to the control embryos; n = 150 centrosomes and k = 3 embryos for each background. Scale bar = 5 μm. All scatter plots show the mean ± SD. Statistical significance was calculated using the Mann–Whitney U-test. ns, not significant. ****P < 0.0001.

Quantitation of centrosomal MTs in CLASP and Patronin embryos reveals an antagonistic relationship between centrosomal and ncMT networks. (A) γ-tubulin:GFP intensities vary in control, Patronin, and CLASP embryos. (B) Quantification of γ-tubulin:GFP in control, Patronin, and CLASP embryos at 0 and 20 min GBE; n = 150 for control and Patronin, and 200 centrosomes for CLASP at 0 min and n = 88, 150 and 172 centrosomes for control, Patronin and CLASP, respectively, at 20 min; control and Patronin k = 3 and CLASP k = 4 embryos. (C) Still images showing the localization of Patronin:GFP in control and CLASP embryos at 0 and 20 min GBE, highlighting the absence of perinuclear Patronin in CLASP embryos. (D) Perinuclear Patronin:GFP intensities in control and CLASP embryos 5 min before GBE onset showing depleted perinuclear intensities in CLASP embryos; n = 75 perinuclear regions from k = 3 embryos for each background. (E) Still images showing Patronin:GFP enrichment at centrosomes (5 μm below cell apices) in CLASP embryos unlike in control embryos (same control as Fig. 5 A and Fig. 7 A, 0 min at 5 μm), arrows mark centrosomal Patronin. (F) Centrosomal Patronin:GFP intensities at 0 and 20 min show upregulated centrosomal Patronin in CLASP compared to the control embryos; n = 150 centrosomes and k = 3 embryos for each background. Scale bar = 5 μm. All scatter plots show the mean ± SD. Statistical significance was calculated using the Mann–Whitney U-test. ns, not significant. ****P < 0.0001.

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