CLASP disruption enhances centrosomal MTs while depleting perinuclear MT networks. (A) Quantification showing depleted MT intensities in apical regions above nuclei in CLASP-compromised embryos as compared to the control at 20 min into GBE; n = 200 regions for control and n = 288 regions for CLASP from k = 3 embryos for each background. (B) Centrosomal MT intensities in CLASP embryos measured at 0 and 20 min GBE; n = 200 centrosomal regions for each time point from k = 4 embryos. (C) Centrosomal MT intensities measurement showing CLASP embryos have further enhancement of centrosomal MTs as compared to Patronin embryos; n = 150 and 200 centrosomal regions for Patronin and CLASP, respectively, at 0 min, and n = 149 and 200 centrosomal regions for Patronin and CLASP, respectively, at 20 min from k = 3 and 4 embryos for Patronin and CLASP, respectively. (D) Images showing CLASP:GFP localization in apical, centrosomal, and perinuclear regions at the onset of GBE. (E) Orthogonal view of acetylated MT color-coded for intensity levels in control and CLASP embryos, highlighting depleted and fragmented MT pools in CLASP embryos. Arrows mark acetylated MT in perinuclear regions. (E′) Quantification of acetylated MT intensities for control and CLASP embryos (mean ± SD); n = 70 regions from k = 7 embryos for each background. Scale bar = 5 μm. All scatter plots show the mean ± SD. Statistical significance was calculated using the Mann–Whitney U-test. ***P < 0.001 and ****P < 0.0001.