Complete T cell antigen alters the DC phenotype of DLN in a CD4 ⁺ T cell–dependent manner. (A) Experimental schedule for CD4⁺ T cell depletion in the MC38 model. (B) Effects of CD4⁺ T cell depletion in the DLN cells on day 20. (C) MC38 tumor volumes following Bpmel-OVAI-Ea treatment with or without CD4⁺ T cell depletion (n = 5 per group). (D) Adpgk^MUT-specific CD8 TIL infiltration (n = 4 per group). Student’s t test (C) and one-way ANOVA followed by Dunnett’s multiple comparison was used (D). (E–G) scRNAseq analysis of DLN cells, excluding naive T cells, from MC38-bearing mice treated with or without Bpmel-SIY-OVAII on day 6 (control: n = 10,952 and SIY-OVAII: n = 18,440 cells); (E) UMAP of the two group-mixture cells; (F) a scatter plot of incoming and outgoing interaction strengths for each population; (G) communication probabilities of the indicated signals in DC of the two groups. The relative information flow is the ratio of the communication probability between the experimental groups. Red and blue (Y axis) indicate significant enrichment in the control and SIY-OVAII DLNs, respectively; all data except E–G are representative of two to three similar experiments. UMAP, uniform manifold approximation and projection.