Figure S1.
OvCa cell lines express varying levels of matriptase and HAI-1 mRNA and protein. (A) Whole cell RNA lysates were prepared from IOSE397 and five OvCa cell lines and analyzed by qPCR for matriptase (ST14) and HAI-1 (SPINT1) mRNA expression. Data represents average mRNA expression relative to GAPDH (error bars are ± SEM from three independent experiments performed in triplicate; *P < 0.05 relative to IOSE397 cells). (B) Whole cell lysates were collected from IOSE397 and five OvCa cell lines with RIPA lysis buffer, assessed by SDS-PAGE, and immunoblotted for rabbit anti-human matriptase, mouse anti-HAI-1, and rabbit anti-human β-tubulin (representative immunoblot shown in left panel). Upper matriptase band (∼90 kD) represents full-length matriptase, while 70 kD fragment represents matriptase zymogen. Densitometric analyses (right panels) were conducted using ImageJ; data represents average densitometric units from three independent experiments (error bars are ± SEM; *P < 0.05, **P < 0.01, ns = not significant relative to IOSE397). (C) Whole-cell RNA lysates were prepared from ES-2-, NCI/ADR-Res, and SKOV3- Vec/Mat cells and analyzed by qPCR for matriptase (ST14) and HAI-1 (SPINT1) mRNA expression. Data represents average mRNA expression relative to GAPDH from three independent experiments performed in triplicate (error bars are ± SEM; *P < 0.05, ****P < 0.001 relative to corresponding Vec cells). (D) Whole-cell lysates were collected from ES-2-, NCI/ADR-Res, and SKOV3- Vec/Mat cells with RIPA lysis buffer, assessed by SDS-PAGE and immunoblotted for rabbit anti-human matriptase, mouse anti-HAI-1, and rabbit anti-human β-tubulin (representative immunoblot shown in left panel). Densitometric analyses (right panels) were conducted using ImageJ; data represents average densitometric units from three independent experiments (errors bars are ± SEM; *P < 0.05, **P < 0.01, ***P < 0.005, ns = not significant relative to respective Vec controls). All P values were calculated according to two-tailed, unpaired Student’s t tests. Source data are available for this figure: SourceData FS1.

OvCa cell lines express varying levels of matriptase and HAI-1 mRNA and protein. (A) Whole cell RNA lysates were prepared from IOSE397 and five OvCa cell lines and analyzed by qPCR for matriptase (ST14) and HAI-1 (SPINT1) mRNA expression. Data represents average mRNA expression relative to GAPDH (error bars are ± SEM from three independent experiments performed in triplicate; *P < 0.05 relative to IOSE397 cells). (B) Whole cell lysates were collected from IOSE397 and five OvCa cell lines with RIPA lysis buffer, assessed by SDS-PAGE, and immunoblotted for rabbit anti-human matriptase, mouse anti-HAI-1, and rabbit anti-human β-tubulin (representative immunoblot shown in left panel). Upper matriptase band (∼90 kD) represents full-length matriptase, while 70 kD fragment represents matriptase zymogen. Densitometric analyses (right panels) were conducted using ImageJ; data represents average densitometric units from three independent experiments (error bars are ± SEM; *P < 0.05, **P < 0.01, ns = not significant relative to IOSE397). (C) Whole-cell RNA lysates were prepared from ES-2-, NCI/ADR-Res, and SKOV3- Vec/Mat cells and analyzed by qPCR for matriptase (ST14) and HAI-1 (SPINT1) mRNA expression. Data represents average mRNA expression relative to GAPDH from three independent experiments performed in triplicate (error bars are ± SEM; *P < 0.05, ****P < 0.001 relative to corresponding Vec cells). (D) Whole-cell lysates were collected from ES-2-, NCI/ADR-Res, and SKOV3- Vec/Mat cells with RIPA lysis buffer, assessed by SDS-PAGE and immunoblotted for rabbit anti-human matriptase, mouse anti-HAI-1, and rabbit anti-human β-tubulin (representative immunoblot shown in left panel). Densitometric analyses (right panels) were conducted using ImageJ; data represents average densitometric units from three independent experiments (errors bars are ± SEM; *P < 0.05, **P < 0.01, ***P < 0.005, ns = not significant relative to respective Vec controls). All P values were calculated according to two-tailed, unpaired Student’s t tests. Source data are available for this figure: SourceData FS1.

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