Figure 7.
RUNX1, CTCF, Med12, p300, and Notch1-IC–binding at the Tcf7 and Gata3 loci in LP and Phase 1 cells. (A) TPM values for Tcf7 and Gata3 in Cbfb-deficient LPs or Phase 1 cells are shown with SDs. The data represent the mean values of three independent biological replicates. The data were analyzed by a two-sided t test. For Tcf7 mRNA, **P = 0.003. For GATA3 mRNA, **P = 0.0001. (B and C) Representative ChIP-seq tracks for RUNX1, CTCF, Med12, and p300 in LP and Phase 1, Notch1-IC in LP, Phase 1, and Phase 2, Med12 in sgControl- or sgCbfb-transduced Phase 1 cells, and RUNX1 in sgControl- or sgMed12-transduced Phase 1 cells around the Tcf7 (B) and Gata3 (C) loci. Phase 1–specific RUNX1/Med12/p300/Notch-IC–binding sites are labeled with red rectangles, including the Tcf7 enhancer (−31 kb) and T cell–specific Gata3 enhancer (+280 kb) regions. Data are representative of two independent experiments. SD, standard deviation.

RUNX1, CTCF, Med12, p300, and Notch1-IC–binding at the Tcf7 and Gata3 loci in LP and Phase 1 cells. (A) TPM values for Tcf7 and Gata3 in Cbfb-deficient LPs or Phase 1 cells are shown with SDs. The data represent the mean values of three independent biological replicates. The data were analyzed by a two-sided t test. For Tcf7 mRNA, **P = 0.003. For GATA3 mRNA, **P = 0.0001. (B and C) Representative ChIP-seq tracks for RUNX1, CTCF, Med12, and p300 in LP and Phase 1, Notch1-IC in LP, Phase 1, and Phase 2, Med12 in sgControl- or sgCbfb-transduced Phase 1 cells, and RUNX1 in sgControl- or sgMed12-transduced Phase 1 cells around the Tcf7 (B) and Gata3 (C) loci. Phase 1–specific RUNX1/Med12/p300/Notch-IC–binding sites are labeled with red rectangles, including the Tcf7 enhancer (−31 kb) and T cell–specific Gata3 enhancer (+280 kb) regions. Data are representative of two independent experiments. SD, standard deviation.

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