Figure S5.
Notch-dependent functional conversion of RUNX TFs in T-lineage commitment. (A) Number of LP-specific RUNX1 peaks co-occupied with CTCF in whole genome or RUNX- and CTCF-repressed among Notch-activated genes (Fig. 6 B) are shown. The data were analyzed using a chi-square test with Yates’s correction. (B) Number of Phase 1–specific RUNX1 peaks co-occupied with Notch1-IC in whole genome or Notch-activated genes are shown. The data were analyzed using a chi-square test with Yates’s correction. (C) Relative percentage of CD19+ cells among CD45+Lin− cells from Fig. 8 A are shown, including their SDs. (D) BM progenitors were cocultured with OP9, and the B cell developmental status was scored using the markers CD19 and Kit among CD45+Lin- cells on day 4. (E) Relative percentage of Lin−CD19− cells among CD45+ cells from Fig. 8 D are shown, including their SDs. Data in C and E represent mean values from three independent biological replicates. Data in D are representative of two independent experiments. The data were analyzed using a one-way ANOVA with Dunnett’s multiple comparisons (C and E). For C: CD19+, ***adjusted P = 0.0007 for sgCbfb; **adjusted P = 0.006 for sgCTCF; ***adjusted P = 0.001 for sgMed12. For E: Lin−, *adjusted P = 0.0145 for sgCbfb; *adjusted P = 0.0116 for sgMed12. SD, standard deviation.

Notch-dependent functional conversion of RUNX TFs in T-lineage commitment. (A) Number of LP-specific RUNX1 peaks co-occupied with CTCF in whole genome or RUNX- and CTCF-repressed among Notch-activated genes (Fig. 6 B) are shown. The data were analyzed using a chi-square test with Yates’s correction. (B) Number of Phase 1–specific RUNX1 peaks co-occupied with Notch1-IC in whole genome or Notch-activated genes are shown. The data were analyzed using a chi-square test with Yates’s correction. (C) Relative percentage of CD19+ cells among CD45+Lin− cells from Fig. 8 A are shown, including their SDs. (D) BM progenitors were cocultured with OP9, and the B cell developmental status was scored using the markers CD19 and Kit among CD45+Lin- cells on day 4. (E) Relative percentage of Lin−CD19− cells among CD45+ cells from Fig. 8 D are shown, including their SDs. Data in C and E represent mean values from three independent biological replicates. Data in D are representative of two independent experiments. The data were analyzed using a one-way ANOVA with Dunnett’s multiple comparisons (C and E). For C: CD19+, ***adjusted P = 0.0007 for sgCbfb; **adjusted P = 0.006 for sgCTCF; ***adjusted P = 0.001 for sgMed12. For E: Lin−, *adjusted P = 0.0145 for sgCbfb; *adjusted P = 0.0116 for sgMed12. SD, standard deviation.

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