RUNX represses T-signature genes in LPs and activates them in Phase 1. (A) Schematic representation of the transcriptome analysis experiment. Retroviral vectors encoding sgRNAs targeting luciferase (sgControl) or Cbfb (sgCbfb) were introduced into Cas9-LPs. 5 days after sgRNA introduction, hNGFR⁺ CD45⁺ LP cells were sorted and subjected to QuantSeq 3′ mRNA sequencing. (B) Venn diagrams show the numbers of RUNX-dependent and RUNX-repressed DEGs in LPs (Notch-) and Notch-stimulated LPs for 2 days (Phase 1; Notch+). (The full list of the DEGs is provided in Table S2.) (C) Heatmaps show the expression changes of representative Notch-activated T-signature genes (Romero-Wolf et al., 2020) associated with T cell development following Cbfb deletion. Data are based on the average of three biological replicates.