Figure S2. RUSH-α5 recruitment to... | Rockefeller University Press

Figure S2.

$RUSH-α5 recruitment to adhesions is ligand-dependent. (A) Representative immunoblot of GFP pulldowns performed in RUSH-α5 or control transfected cells plated on FN or collagen and probed for endogenous integrin β1 and for GFP. Mature (magenta arrow) and immature (green arrow) integrin β1 are indicated. (B) Quantification of the relative fraction of mature to immature integrin β1 interacting with RUSH-α5 ± biotin treatment for the indicated times. N = 6 independent experiments; data are mean ± SD, One-way ANOVA, Dunn’s multiple comparison test, no significant difference between FN and collagen at all time points. (C) Representative images (see Video 5) of U2OS cells co-expressing RUSH-α5 and RUSH-CD59 and plated on FN (top) or collagen (bottom) ± biotin treatment for the indicated times. Insets represent ROIs that are magnified. Scale bars: 20 µm. Source data are available for this figure: SourceData FS2.$

RUSH-α5 recruitment to adhesions is ligand-dependent. (A) Representative immunoblot of GFP pulldowns performed in RUSH-α5 or control transfected cells plated on FN or collagen and probed for endogenous integrin β1 and for GFP. Mature (magenta arrow) and immature (green arrow) integrin β1 are indicated. (B) Quantification of the relative fraction of mature to immature integrin β1 interacting with RUSH-α5 ± biotin treatment for the indicated times. N = 6 independent experiments; data are mean ± SD, One-way ANOVA, Dunn’s multiple comparison test, no significant difference between FN and collagen at all time points. (C) Representative images (see Video 5) of U2OS cells co-expressing RUSH-α5 and RUSH-CD59 and plated on FN (top) or collagen (bottom) ± biotin treatment for the indicated times. Insets represent ROIs that are magnified. Scale bars: 20 µm. Source data are available for this figure: SourceData FS2.

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