Macrophage depletion and erythrocyte enucleation in anti-CSF1R antibody-treated embryos. (A) Representative flow cytometry plots and total cell numbers (per mg tissue) (mean ± SD) of macrophages (pre-gated on CD45⁺Ly6G⁻ cells) from livers of anti-CSF1R antibody-treated E16.5 embryos. Data show n = 5–6 samples per group and are representative of two independent experiments. Statistical analysis was performed using the Student’s t test. (B) Microscopic images of peripheral blood smears and quantification of nucleated RBC (%) (mean ± SD) from untreated (Ctrl) and anti-CSF1R antibody-treated E15.5 embryos stained with Giemsa. Scale bar is 40 µm. Data show n = 4 samples per group and are representative of two independent experiments. Statistical analysis was performed using the Student’s t test. (C) Analysis of peripheral blood parameters from untreated (Ctrl) and anti-CSF1R antibody-treated E15.5 embryos using a blood counter. Data shown are n = 5–6 samples per group and are representative of two independent experiments. Statistical analysis was performed using the Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. ns, not significant; RBC, red blood cells; HGB, hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; RDW, red cell distribution width.