XPR1 is required for the induction of the KC transcriptional program. (A) UMAP plot and annotation of clusters of CD45⁺Lin⁻ (CD19⁻CD3⁻B220⁻Ter119⁻CD49b⁻CD90.2⁻) cells extracted from E15.5 Xpr1^fl/fl and Vav1^iCreXpr1^fl/fl livers, analyzed by scRNA-seq. Data were generated from n = 3 samples per genotype. (B) UMAP plots, guided clustering, and manual cell annotation of subsetted data from the scRNA-seq for clusters 0, 1, 2, 3, 10, and 13 in A. (C) Violin plots showing liver macrophage gene expression signature (Bonnardel et al., 2019) among clusters 2, 3, 6, and 7 of the scRNA-seq data in B. (D) Violin plots showing monocyte gene expression signature (Bonnardel et al., 2019) among clusters 2, 3, 6, and 7 of the scRNA-seq data in B. (E) Violin plots showing the expression of liver macrophage–associated transcription factors among clusters 0–8 of the scRNA-seq data in B.