Figure S2.
Xpr1 expression in adult and embryonic liver cell populations. (A) Violin plots showing the expression of Xpr1 in indicated adult mouse liver cell populations. Data from Guilliams et al. (2022). (B) Violin plots showing the expression of Xpr1 in indicated E16 embryonic liver cell populations. Data from Wang et al. (2020). (C) Graph showing the Mendelian distribution (and numbers) of E15.5–E18.5 embryos from a Vav1iCreXpr1fl/fl × Xpr1fl/fl breeding. (D) Representative flow cytometry plots of FL macrophages from E16.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl embryos (pre-gated on CD45+ cells). Related to Fig. 2 A. (E) Representative flow cytometry plots and total cell numbers (mean ± SD) of YS EMPs and macrophages extracted from E10.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl embryos (pre-gated on CD45+ cells). n = 6–8 per group. Statistical analysis was performed using the Student’s t test. (F) Flow cytometry plots showing the gating strategy used to sort endothelial cells (CD45−Lyve1+) and Ly6Chi monocytes from E16.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl FLs. (G) Graphs showing Xpr1 expression in sorted endothelial cells and Ly6Chi monocytes (as in F) extracted from livers of E16.5 control (Xpr1fl/fl and Xpr1fl/+) and Vav1iCreXpr1fl/fl embryos. Data shown are n = 3 per group and are representative of two independent experiments. Statistical analysis was performed using the Student’s t test. (H) Endothelial cell numbers enumerated from E15.5 control (Xpr1fl/fl and Xpr1fl/+) and Vav1iCreXpr1fl/fl embryos using flow cytometry (gated on CD31+CD45− cells). Data shown are n = 3–5 per group and are representative of two independent experiments. Statistical analysis was performed using the Student’s t test. (I) Representative flow cytometry plots of FL monocytes extracted from E16.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl embryos (pre-gated on CD45+Lin−F4/80−CD117−Ly6G−Ly6C+ cells). Related to Fig. 2 F. (J and K) Fluorescent images of E15.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl FLs stained with DAPI, TUNEL and CD64. (J) Representative images, scale bars: 50 µm. (K) TUNEL mean staining intensity was quantified within the CD64+ area of E15.5 control (Xpr1fl/fl and Xpr1fl/+) and Vav1iCreXpr1fl/fl FLs. Data shown are n = 4 per group and statistical analysis was performed using the Student’s t test. **P < 0.01 and ***P < 0.001. ns, not significant.

Xpr1 expression in adult and embryonic liver cell populations. (A) Violin plots showing the expression of Xpr1 in indicated adult mouse liver cell populations. Data from Guilliams et al. (2022). (B) Violin plots showing the expression of Xpr1 in indicated E16 embryonic liver cell populations. Data from Wang et al. (2020). (C) Graph showing the Mendelian distribution (and numbers) of E15.5–E18.5 embryos from a Vav1iCreXpr1fl/fl × Xpr1fl/fl breeding. (D) Representative flow cytometry plots of FL macrophages from E16.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl embryos (pre-gated on CD45+ cells). Related to Fig. 2 A. (E) Representative flow cytometry plots and total cell numbers (mean ± SD) of YS EMPs and macrophages extracted from E10.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl embryos (pre-gated on CD45+ cells). n = 6–8 per group. Statistical analysis was performed using the Student’s t test. (F) Flow cytometry plots showing the gating strategy used to sort endothelial cells (CD45−Lyve1+) and Ly6Chi monocytes from E16.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl FLs. (G) Graphs showing Xpr1 expression in sorted endothelial cells and Ly6Chi monocytes (as in F) extracted from livers of E16.5 control (Xpr1fl/fl and Xpr1fl/+) and Vav1iCreXpr1fl/fl embryos. Data shown are n = 3 per group and are representative of two independent experiments. Statistical analysis was performed using the Student’s t test. (H) Endothelial cell numbers enumerated from E15.5 control (Xpr1fl/fl and Xpr1fl/+) and Vav1iCreXpr1fl/fl embryos using flow cytometry (gated on CD31+CD45− cells). Data shown are n = 3–5 per group and are representative of two independent experiments. Statistical analysis was performed using the Student’s t test. (I) Representative flow cytometry plots of FL monocytes extracted from E16.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl embryos (pre-gated on CD45+Lin−F4/80−CD117−Ly6G−Ly6C+ cells). Related to Fig. 2 F. (J and K) Fluorescent images of E15.5 Xpr1fl/fl and Vav1iCreXpr1fl/fl FLs stained with DAPI, TUNEL and CD64. (J) Representative images, scale bars: 50 µm. (K) TUNEL mean staining intensity was quantified within the CD64+ area of E15.5 control (Xpr1fl/fl and Xpr1fl/+) and Vav1iCreXpr1fl/fl FLs. Data shown are n = 4 per group and statistical analysis was performed using the Student’s t test. **P < 0.01 and ***P < 0.001. ns, not significant.

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