Figure S1.
Lung, kidney, and heart macrophages are not affected in Xpr1LacZ/LacZmice. (A) Schematic showing the Xpr1LacZ allele and how crossing it to a Flp-deleter results in an Xpr1fl/fl allele. (B) Graph showing the Mendelian distribution (and numbers) of E13.5–E18.5 embryos from an Xpr1LacZ/+ × Xpr1LacZ/+ crossing. (C) Graph showing the total numbers (mean ± SD) of microglia and BAMs extracted from Xpr1+/+, Xpr1LacZ/+, and Xpr1LacZ/LacZ embryos at indicated time points, analyzed by flow cytometry (as in Fig. 1 A). Each time point shows data from, n = 7–16, pooled from at least two independent experiments. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test. (D) Total cell numbers (mean ± SD) of LC precursors and Ly6Chi monocytes extracted from limb buds (E14.5) or skin (epidermis and dermis) of E16.5 Xpr1+/+, Xpr1LacZ/+, and Xpr1LacZ/LacZ embryos, analyzed by flow cytometry. Each time point shows data from at least two independent experiments, n = 5–9 per group and time point. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test. Related to Fig. 1 B. (E) Graph showing the total numbers (mean ± SD) of FL macrophages extracted from control (Xpr1+/+ and Xpr1LacZ/+) and Xpr1LacZ/LacZ embryos at E12.5, analyzed by flow cytometry. Data shown are n = 4 per group and are representative of two independent experiments. Statistical analysis was performed using the Student’s t test. Related to Fig. 1 C. (F) Graphs showing the weights of livers (mean ± SD) extracted from Xpr1+/+, Xpr1LacZ/+, and Xpr1LacZ/LacZ embryos at indicated time points. Data shown are at least n = 3 per genotype and time point and are representative of at least two independent experiments. Statistical analysis was performed using the Student’s t test. (G) Representative flow cytometry plots, frequencies and total numbers (mean ± SD) of cells extracted from hearts, kidneys, and lungs of Xpr1+/+, Xpr1LacZ/+, and Xpr1LacZ/LacZ embryos (pre-gated on Live CD45+Ly6C−Ly6G− cells) as indicated. Each time point shows data from at least two independent experiments, n = 5–16 per group and time point. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, and ****P < 0.0001. ns, not significant.

Lung, kidney, and heart macrophages are not affected in Xpr1 LacZ/LacZ mice. (A) Schematic showing the Xpr1LacZ allele and how crossing it to a Flp-deleter results in an Xpr1fl/fl allele. (B) Graph showing the Mendelian distribution (and numbers) of E13.5–E18.5 embryos from an Xpr1LacZ/+ × Xpr1LacZ/+ crossing. (C) Graph showing the total numbers (mean ± SD) of microglia and BAMs extracted from Xpr1+/+, Xpr1LacZ/+, and Xpr1LacZ/LacZ embryos at indicated time points, analyzed by flow cytometry (as in Fig. 1 A). Each time point shows data from, n = 7–16, pooled from at least two independent experiments. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test. (D) Total cell numbers (mean ± SD) of LC precursors and Ly6Chi monocytes extracted from limb buds (E14.5) or skin (epidermis and dermis) of E16.5 Xpr1+/+, Xpr1LacZ/+, and Xpr1LacZ/LacZ embryos, analyzed by flow cytometry. Each time point shows data from at least two independent experiments, n = 5–9 per group and time point. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test. Related to Fig. 1 B. (E) Graph showing the total numbers (mean ± SD) of FL macrophages extracted from control (Xpr1+/+ and Xpr1LacZ/+) and Xpr1LacZ/LacZ embryos at E12.5, analyzed by flow cytometry. Data shown are n = 4 per group and are representative of two independent experiments. Statistical analysis was performed using the Student’s t test. Related to Fig. 1 C. (F) Graphs showing the weights of livers (mean ± SD) extracted from Xpr1+/+, Xpr1LacZ/+, and Xpr1LacZ/LacZ embryos at indicated time points. Data shown are at least n = 3 per genotype and time point and are representative of at least two independent experiments. Statistical analysis was performed using the Student’s t test. (G) Representative flow cytometry plots, frequencies and total numbers (mean ± SD) of cells extracted from hearts, kidneys, and lungs of Xpr1+/+, Xpr1LacZ/+, and Xpr1LacZ/LacZ embryos (pre-gated on Live CD45+Ly6C−Ly6G− cells) as indicated. Each time point shows data from at least two independent experiments, n = 5–16 per group and time point. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, and ****P < 0.0001. ns, not significant.

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