Slow tail currents and rates of trypsin digestion distinguish between channels inactivated by β2 and β3a. (A) BK+β2 currents at the indicated times of cumulative trypsin digestion. (B) BK+mβ3a currents during trypsin digestion. (C) Changes in steady-state current amplitude (fss) at different times of trypsin digestion. Means ± SD. Curves were fit with the indicated function, yielding the indicated time constants of digestion. N = number of patches. (D) Changes in normalized slow tail amplitude (AST) as a function of fss measured at different times of trypsin digestion from 5 patches for β3a and 5 patches for β2. For BK+β3a, peak slow tail amplitude prior to trypsin was used to normalize subsequent amplitudes measured during trypsin digestion. For BK+ β2, initial tail current amplitude was measured at t = 3 ms after nominal repolarization, and minimal changes were observed with digestion.
Figure 2.

Slow tail currents and rates of trypsin digestion distinguish between channels inactivated by β2 and β3a. (A) BK+β2 currents at the indicated times of cumulative trypsin digestion. (B) BK+mβ3a currents during trypsin digestion. (C) Changes in steady-state current amplitude (fss) at different times of trypsin digestion. Means ± SD. Curves were fit with the indicated function, yielding the indicated time constants of digestion. N = number of patches. (D) Changes in normalized slow tail amplitude (AST) as a function of fss measured at different times of trypsin digestion from 5 patches for β3a and 5 patches for β2. For BK+β3a, peak slow tail amplitude prior to trypsin was used to normalize subsequent amplitudes measured during trypsin digestion. For BK+ β2, initial tail current amplitude was measured at t = 3 ms after nominal repolarization, and minimal changes were observed with digestion.

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