Molecular characterization of lymph node FRCs and VSMCs. (A and B) scRNA-seq data of VSMCs and FRCs from peripheral and mesenteric lymph nodes of 8-wk-old Ccl19-iEYFP mice. (A) UMAP representation split by lymph node location and colored by subset identity. (B) Relative abundance of FRC subsets and VSMCs in peripheral and mesenteric murine lymph nodes of Ccl19-iEYFP mice. (C and D) scRNA-seq data of VSMCs and FRCs from peripheral and mesenteric lymph nodes of 8-wk-old Cxcl13-EYFP mice. (C) UMAP representation split by lymph node location and colored by subset identity. (D) Relative abundance of FRC subsets and VSMCs in peripheral and mesenteric murine lymph nodes of Cxcl13-EYFP mice. (E) Dot plot indicating the average expression of signature genes across VSMCs and FRC subsets in lymph nodes of Ccl19-iEYFP mice. (F) Dot plot indicating the average expression of signature genes across VSMCs and FRC subsets in lymph nodes of Cxcl13-EYFP mice. (G and H) Differentially expressed gene analysis between FRCs and VSMCs isolated from peripheral and mesenteric lymph nodes. (G) Enriched gene sets based on differentially expressed genes in peripheral and mesenteric lymph nodes. (H) Violin plots showing gene expression profiles of selected differentially expressed genes. Lymph node scRNA-seq data of Ccl19-iEYFP mice are representative of n = 15 mice from four independent experiments; 52,188 cells in total. Lymph node scRNA-seq data of Cxcl13-EYFP mice are representative of n = 10 mice from two independent experiments; 22,288 cells in total.