Figure S1.
Transgene activity in peripheral and mesenteric lymph nodes of Ccl19-iEYFP and Cxcl13-EYFP mice. (A) Schematic representation of the triple transgenic Ccl19-iEYFP mouse model. (B) Confocal microscopy images of cross sections of representative inguinal and mesenteric lymph nodes from 8-wk-old Ccl19-Cre (Ccl19-EYFP) mice. Microscopy images are representative for three inguinal and three mesenteric lymph nodes from two independent experiments. Scale bars: 200 µm (left panels) and 300 µm (right panels). (C–F) Confocal microscopy images showing current Ccl19 promoter activity (TdTomato expression) in cross sections of inguinal (C and D) and mesenteric lymph nodes (E and F) from Ccl19-iEYFP mice. Boxes indicate regions of higher magnification. Microscopy images are representative for three inguinal and three mesenteric lymph nodes from two independent experiments. Scale bars: 200 µm, 500 µm (C and E, overviews) and 20 µm (D and F, zoom-ins). (G and H) Confocal microscopy images showing venules at higher magnification in inguinal (G) and mesenteric (H) lymph nodes from Ccl19-iEYFP mice. Arrows indicate the connection of VSMCs to the FRC network by PDPN+ PRCs. Microscopy images are representative for three inguinal and three mesenteric lymph nodes from two independent experiments. Scale bars: 20 µm. (I) Confocal microscopy images showing blood vessels (BV) at high magnification surrounded by ACTA2+ VSMCs and CD34+ PRCs. (J–N) Confocal microscopy images of inguinal and mesenteric lymph nodes from Cxcl13-EYFP mice. (J) Confocal microscopy images of cross sections of representative inguinal lymph nodes from 8-wk-old Cxcl13-EYFP mice. (K) Confocal microscopy images of higher magnified B cell follicles with TdTomato indicating CXCL13 expression. (L) Confocal microscopy images of cross sections of representative mesenteric lymph nodes from 8-wk-old Cxcl13-EYFP mice. (M and N) Confocal microscopy images showing venules at higher magnification in inguinal (M) and mesenteric lymph nodes (N) from Cxcl13-EYFP mice. Microscopy images are representative for three inguinal and three mesenteric lymph nodes from three independent experiments. Overviews in B, C, E, J, and L are stitched images from tile scans. Scale bars: 200 µm (J), 50 µm (K), 500 µm (L), 20, and 10 µm (M and N).

Transgene activity in peripheral and mesenteric lymph nodes of Ccl19-iEYFP and Cxcl13-EYFP mice. (A) Schematic representation of the triple transgenic Ccl19-iEYFP mouse model. (B) Confocal microscopy images of cross sections of representative inguinal and mesenteric lymph nodes from 8-wk-old Ccl19-Cre (Ccl19-EYFP) mice. Microscopy images are representative for three inguinal and three mesenteric lymph nodes from two independent experiments. Scale bars: 200 µm (left panels) and 300 µm (right panels). (C–F) Confocal microscopy images showing current Ccl19 promoter activity (TdTomato expression) in cross sections of inguinal (C and D) and mesenteric lymph nodes (E and F) from Ccl19-iEYFP mice. Boxes indicate regions of higher magnification. Microscopy images are representative for three inguinal and three mesenteric lymph nodes from two independent experiments. Scale bars: 200 µm, 500 µm (C and E, overviews) and 20 µm (D and F, zoom-ins). (G and H) Confocal microscopy images showing venules at higher magnification in inguinal (G) and mesenteric (H) lymph nodes from Ccl19-iEYFP mice. Arrows indicate the connection of VSMCs to the FRC network by PDPN+ PRCs. Microscopy images are representative for three inguinal and three mesenteric lymph nodes from two independent experiments. Scale bars: 20 µm. (I) Confocal microscopy images showing blood vessels (BV) at high magnification surrounded by ACTA2+ VSMCs and CD34+ PRCs. (J–N) Confocal microscopy images of inguinal and mesenteric lymph nodes from Cxcl13-EYFP mice. (J) Confocal microscopy images of cross sections of representative inguinal lymph nodes from 8-wk-old Cxcl13-EYFP mice. (K) Confocal microscopy images of higher magnified B cell follicles with TdTomato indicating CXCL13 expression. (L) Confocal microscopy images of cross sections of representative mesenteric lymph nodes from 8-wk-old Cxcl13-EYFP mice. (M and N) Confocal microscopy images showing venules at higher magnification in inguinal (M) and mesenteric lymph nodes (N) from Cxcl13-EYFP mice. Microscopy images are representative for three inguinal and three mesenteric lymph nodes from three independent experiments. Overviews in B, C, E, J, and L are stitched images from tile scans. Scale bars: 200 µm (J), 50 µm (K), 500 µm (L), 20, and 10 µm (M and N).

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