Gating strategies of flow cytometry cell line experiments. (A and B) Shown are the gating strategies used in (A) MR1 upregulation experiments and (B) T cell activation experiments. (C–K) Bar graphs show three independent biological replicates for the CD69 expression measured on the surface of SKW-3 overexpressing the typical MAIT cell clones AF-7 (TRAV1-2+ TRBV6-1+) cocultured with C1R.MR1 cells in the absence (C–E) or presence of RL-7-Me (F–H) or 5-OP-RU (I–K) after preincubation with titrated quantities of indicated ligands. Individual data points in C–K are technical replicates within the same experiment.
Figure S2.

Gating strategies of flow cytometry cell line experiments. (A and B) Shown are the gating strategies used in (A) MR1 upregulation experiments and (B) T cell activation experiments. (C–K) Bar graphs show three independent biological replicates for the CD69 expression measured on the surface of SKW-3 overexpressing the typical MAIT cell clones AF-7 (TRAV1-2+ TRBV6-1+) cocultured with C1R.MR1 cells in the absence (C–E) or presence of RL-7-Me (F–H) or 5-OP-RU (I–K) after preincubation with titrated quantities of indicated ligands. Individual data points in C–K are technical replicates within the same experiment.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal