Characterization of Cbs ^−/− mice. (A) Schematic illustration of the Cbs gene targeting strategy (top) and western blot analysis of CBS protein in Cbs^−/− mice (bottom). (B) Tissue-specific expression pattern of Cbs gene in mice revealed by transcriptome analysis. The RNA-seq data (n = 18 tissues) were generated by the Mouse ENCODE project PRJNA66167. The transcripts per million (TPM) values are presented as bar graph. (C–E) Comparison of kidneys (C), spleens (D), and brains (E) between WT and Cbs^−/− mice. Representative images are shown on the left, and quantifications are shown on the right. Sample sizes (number of mice) are shown for each group. Bars, 2 mm. (F and G) Oil Red O staining of sections of kidneys (F) and spleens (G) from WT and Cbs^−/− mice. Representative images are shown on the left, and quantifications are shown on the right. Bars, 50 μm. Sample sizes (number of mice) are shown for each group. (H and I) H&E staining of sections of kidneys (F) and spleens (G) from WT and Cbs^−/− mice. Representative images are shown on the left, and quantifications are shown on the right. Bars, 50 μm. Sample sizes (number of mice) are shown for each group. For all quantifications, data are presented as mean ± SEM. Statistical significance was calculated using the two-sided Student’s t test. ns, not significant. Source data are available for this figure: SourceData FS5.