C. violaceum infection activates TFEB. (A–E) Immunoblot analysis demonstrating TFEB activation following 4-h infection with WT C. violaceum (C.v. WT) in (A) KCs (MOI = 2), (B) HSCs (MOI = 5), (C) HepG2 cells (MOI = 5), (D) Huh7 cells (MOI = 5), and (E) AML12 cells (MOI = 5). Positive controls: cells were treated with DMSO or Torin1 (330 nM) for 4 h. All lysates were analyzed by immunoblotting with anti-TFEB antibody. Source data are available for this figure: SourceData FS4.