Schematic of tensin3 TBS binding to the autoinhibited talin1. Superposition of the NMR-validated AlphaFold3 models of the talin R3, R4, and R8 (yellow), and crystal structure of the talin R11 (purple) in complex with tensin3 TBS peptide (red) on the cryoEM structure of the autoinhibited form of the full-length talin1 (PDB ID: 8VDQ, gray). The superposition was performed using the align function of PyMOL. Note that in the autoinhibited talin1, the R3, R8, and R11 domains are fully exposed to tensin3 TBS, whereas the tensin3 binding to R4 (highlighted by the red dashed circle) is interfered by the talin R1 domain.