SLC7A8 regulates the metabolic fitness of Th2 cells. (A and B) Representative histogram and GMI (Geometric Mean Intensity) quantification of pS6 expression in HDM-instilled lung Th2 cells from R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice. (C–H) Representative histogram and GMI of MitoTracker Green and c-Myc expression in Th2 cells generated in vitro (C, D, F, and G) and in vivo (E and H) from R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice. (I and J) IL-5⁺ Th2 cells were sort purified from the lungs of R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice following administration of HDM and subjected to RNA-seq analysis. Heatmap showing the differentially expressed genes (I) and GO analysis of pathways associated with differentially expressed genes (J). (K and L) Overlaid histograms and quantification showing C-11 BODIPY staining in HDM instilled lung CD4 T cells of from R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice. Data are pooled or representative of two individual experiments n = 3–5 mice per group. Each dot represents an individual mouse. Data are plotted as means ± SEM. Statistics were calculated by unpaired two-tailed Student’s t test. *P < 0.05.