SLC7A8 cell intrinsically regulate Th2 cell function. (A) Naïve Th cells from spleens of R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice were cultured in Th2 conditions. (B–E) Representative FACS plot and quantification of IL-5⁺ and Gata-3⁺Th2 cells. (F) Frequency of Ki67⁺R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl Th2 cells. (G and H) IL-13 production by R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl Th2 cells upon stimulation with PMA/ionomycin. Data are pooled from two individual experiments with n = 1–2 per group. Each dot represents an individual mouse. Data are plotted as means ± SEM. Statistics were calculated by unpaired two-tailed Student’s t test. *P < 0.05 and **P < 0.01, ***P < 0.001.