Tfh cells are comparable between R5 ^Cre Slc7a8 ^+/+ and R5 ^Cre Slc7a8 ^fl/fl mice in the lungs upon HDM-induced allergy. (A) Frequency of IL-5⁺ Th2 cells in the lungs of PBS-instilled R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice. (B and C) Representative FACS plot showing the staining of Tfh (B) and (C) frequency of Tfh in the mediastinal LNs of R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice. (D and E) Representative FACS plot and quantification of IL-5⁺ CD4 T cells after 4 days of culture in Th2 condition. Data are pooled or representative of two individual experiments with n = 3–5 mice per group. Each dot represents an individual mouse. Data are plotted as means ± SEM. Statistics were calculated by unpaired two-tailed Student’s t test. NS, not significant.