Th2 response is impaired in R5 ^Cre Slc7a8 ^fl/fl mice. (A) R5 ^Cre Slc7a8 ^+/+ and R5^CreSlc7a8^fl/fl mice were intranasally instilled with six doses of 50 µg HDM/mouse, and various subsets of Th cells were analyzed. (B and C) Representative FACS plot and quantification of GATA3⁺ Th2 cells from the lungs of R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice. (D–F) Representative FACS plots and quantification of IL-5⁺ Th2 cells from the lungs and mediastinal LNs (MedLN) of R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice. (G)R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice were intranasally instilled with seven doses of 40 µg papain/mouse on indicated days, and Th2 cells were analyzed. (H–J) Representative FACS plots and quantification of IL-5⁺ Th2 cells and GATA3⁺ CD4 T cells from the lungs of R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice. (K)R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice were orally gavaged with larvae of H. polygyrus. (L–N) Egg burden in the feces (L) and GATA3⁺ (M) and IL-5⁺ (N) Th2 cells in the MLN of H. polygyrus–infected R5^CreSlc7a8^+/+ and R5^CreSlc7a8^fl/fl mice. Data are pooled or representative of two individual experiments n = 3–5 mice per group. Each dot represents an individual mouse. Data are plotted as means ± SEM. Statistics were calculated by unpaired two-tailed Student’s t test for all the panels and Mann–Whitney U test for panel I. *P < 0.05 and **P < 0.01.