Slc7a8 is dispensable for T cell development and peripheral homeostasis. (A) Expression of Slc7a8 in splenic T cells upon anti-CD3 and anti-CD28 stimulation in vitro. Frequencies of both CD4⁺ and CD8⁺ T cells were analyzed in the thymus, spleen, and lungs of Il7r^CreSlc7a8^+/+ and Il7r^CreSlc7a8^fl/fl mice. (B) Representative FACS plot showing the staining of CD4 and CD8 in thymus of Il7r^CreSlc7a8^+/+ and Il7r^CreSlc7a8^fl/fl mice. (C–E) Percentage of double-positive (CD4⁺ CD8⁺) (C), CD4⁺ (D), and CD8⁺ (E) T cells in the thymus of Il7r^CreSlc7a8^+/+ and Il7r^CreSlc7a8^fl/fl mice. (F–I) Frequency of CD4⁺ (F and H) and CD8⁺ (G and I) T cells of spleen and lungs of Il7r^CreSlc7a8^+/+ and Il7r^CreSlc7a8^fl/fl mice. Data are pooled or representative of two individual experiments with n = 3–5 mice per group. Each dot represents an individual mouse. Data are plotted as means ± SEM. Statistics were calculated by unpaired two-tailed Student’s t test. NS, not significant. *P < 0.05.