SLC7A8 is specifically expressed in Th2 cells. (A) Expression of Slc7a8 in different subsets of CD4 T cells. (B) Fold induction of Slc7a8 and other transporters in H. polygyrus–induced Th cells as compared with naïve Th cells. (C) qPCR data depicting the expression of Slc7a8 and Slc7a5 in different subset of Th cells. (D–G)Il7r^CreSlc7a8^+/+ and Il7r^CreSlc7a8^fl/fl mice were intranasally instilled with HDM and different subsets of Th cells were scored. (D) Representative FACS plot showing the staining of GATA3 and RORγT in CD4⁺ T cells (pre-gated on CD3⁺, CD4⁺, FOXP3⁻, and Tbet⁻) from the lungs of Il7r^CreSlc7a8^+/+ and Il7r^CreSlc7a8^fl/fl mice. (E) Frequency and numbers of Th2 cells from the lungs of Il7r^CreSlc7a8^+/+ and Il7r^CreSlc7a8^fl/fl mice. (F) Representative figure depicting the eosinophils in the lungs of Il7r^CreSlc7a8^+/+ and Il7r^CreSlc7a8^fl/fl mice. (G) Frequency and number of eosinophils in the lungs of Il7r^CreSlc7a8^+/+ and Il7r^CreSlc7a8^fl/fl mice. Data are pooled or representative of two individual experiments with n = 3–5 mice per group. Each dot represents an individual mouse. Data are plotted as means ± SEM. Statistics were calculated by one-way ANOVA (C) and unpaired two-tailed Student’s t test (E and G). *P < 0.05 and **P < 0.01.