Related toFigs. 6 and 7. (A) Quantification of senGC-dependent secretion in mice prior (day [d]0), during (d1–2), or after (d3–4) exposure to DSS in drinking water by ex vivo mucus growth stimulated with P3CSK4 or carbachol (CCh). (B) Quantification of endocytotic GCs in fixed colon whole mounts of the same mice analyzed in A. (C) Volcano plot of bulk mRNA sequencing data illustrating differential gene expression between ConvD 3-wk (w) and ConvD 2w colonic tissue. (D) Representative micrographs of colonic tissue sections from Duox2fl/fl and Duox2ΔIEC mice after immunohistochemical staining for Muc2. Data represent n = 3–4 animals per group. All data are pooled from at two independent experiments. Statistical analysis by two-way (A) or one-way (B) ANOVA and Fisher’s LSD test; P < 0.01 (**), P < 0.001 (***), P < 0.0001 (****). Image scale bars are 100 µm.
Figure S5.

R elated to Figs. 6 and 7. (A) Quantification of senGC-dependent secretion in mice prior (day [d]0), during (d1–2), or after (d3–4) exposure to DSS in drinking water by ex vivo mucus growth stimulated with P3CSK4 or carbachol (CCh). (B) Quantification of endocytotic GCs in fixed colon whole mounts of the same mice analyzed in A. (C) Volcano plot of bulk mRNA sequencing data illustrating differential gene expression between ConvD 3-wk (w) and ConvD 2w colonic tissue. (D) Representative micrographs of colonic tissue sections from Duox2fl/fl and Duox2ΔIEC mice after immunohistochemical staining for Muc2. Data represent n = 3–4 animals per group. All data are pooled from at two independent experiments. Statistical analysis by two-way (A) or one-way (B) ANOVA and Fisher’s LSD test; P < 0.01 (**), P < 0.001 (***), P < 0.0001 (****). Image scale bars are 100 µm.

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