Plasma membrane depolarization induces protrusions and guides the migration of neutrophils. (A) Schematic of the ion selectivity of CoChR. (B) Time-lapse of ASAP3 and mCherry after photo stimulation using Tg(lyzC: ASAP3;lyzC: CoChR-mCherry)pu42. The ASAP/mCherry ratio is calculated to reflect the transient depolarization. (C) Representative time-lapse images of neutrophil movement before and after the laser stimulation on the indicated subcellular areas using Tg(lyzC: CoChR-mCherry)pu42. The blue laser cartoon indicates the photo-stimulation frame. White arrowheads indicate the direction of cell movement. White boxes indicate the area stimulated. Orange arrowheads represent multiple protrusions. Green arrowheads represent the de novo protrusion. Scale bar, 5 μm. N = 10 cells for each condition.
Figure 4.

Plasma membrane depolarization induces protrusions and guides the migration of neutrophils. (A) Schematic of the ion selectivity of CoChR. (B) Time-lapse of ASAP3 and mCherry after photo stimulation using Tg(lyzC: ASAP3;lyzC: CoChR-mCherry)pu42. The ASAP/mCherry ratio is calculated to reflect the transient depolarization. (C) Representative time-lapse images of neutrophil movement before and after the laser stimulation on the indicated subcellular areas using Tg(lyzC: CoChR-mCherry)pu42. The blue laser cartoon indicates the photo-stimulation frame. White arrowheads indicate the direction of cell movement. White boxes indicate the area stimulated. Orange arrowheads represent multiple protrusions. Green arrowheads represent the de novo protrusion. Scale bar, 5 μm. N = 10 cells for each condition.

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