Kir7.1 regulates zebrafish neutrophil chemotaxis. (A and B) Representative images and quantification (B) for neutrophil response to LTB4 in the Jaguar or WT sibling controls. Scale bar: 50 μm. n > 20 in each group. Data representative of three independent experiments. (C and D) Representative images and quantification (D) for neutrophil response to tail wounding in the Jaguar or WT sibling controls. Scale bar: 50 μm. n > 20 in each group. Data representative of three independent experiments. (E) Representative tracks of neutrophil migration to the ventral fin in response to LTB4 in the Jaguar or WT sibling controls. Each track represents one neutrophil. Scale bar: 50 μm. (F–H) Quantification of the speed (F), directness (G), and forward migration index (H). n >20 in each group. Data representative of three independent experiments. (I) Construct design for neutrophil-specific kcnj13 WT and Q153H mutation overexpression in zebrafish to generate Tg(lyzC:kcnj13-2A-mCherry)pu44 and Tg(lyzC:kcnj13-Q153H-2A-mCherry)pu45. (J and K) Representative images and (K) quantifications of neutrophil recruitment to the tail fin wound. Scale bar, 300 μm. n > 20 in each group. Data representative of three independent experiments. (L and M) Representative tracks and (M) speed of neutrophils’ spontaneous migration in the head mesenchyme in transgenic lines with neutrophil-specific Kir7.1 Q153H overexpression; Tg(lyzC:mCherry)pu35-expressing mCherry alone is used as a control. Scale bar: 50 μm. n > 15 neutrophils from 3 fish of each group. (N and O) Representative images and (O) quantification of the number of neutrophils recruited to the ventral fin upon LTB4 treatment in the indicated zebrafish transgenic lines overexpressing mCherry or Kir7.1Q153H. Scale bar: 50 μm. n > 20 in each group. Data representative of three independent experiments. (B, D, F–H, M, and O) Results are presented as mean ± SD, Mann–Whitney test. (K) Multiple comparisons and Kruskal–Wallis test.
Figure 2.

Kir7.1 regulates zebrafish neutrophil chemotaxis. (A and B) Representative images and quantification (B) for neutrophil response to LTB4 in the Jaguar or WT sibling controls. Scale bar: 50 μm. n > 20 in each group. Data representative of three independent experiments. (C and D) Representative images and quantification (D) for neutrophil response to tail wounding in the Jaguar or WT sibling controls. Scale bar: 50 μm. n > 20 in each group. Data representative of three independent experiments. (E) Representative tracks of neutrophil migration to the ventral fin in response to LTB4 in the Jaguar or WT sibling controls. Each track represents one neutrophil. Scale bar: 50 μm. (F–H) Quantification of the speed (F), directness (G), and forward migration index (H). n >20 in each group. Data representative of three independent experiments. (I) Construct design for neutrophil-specific kcnj13 WT and Q153H mutation overexpression in zebrafish to generate Tg(lyzC:kcnj13-2A-mCherry)pu44 and Tg(lyzC:kcnj13-Q153H-2A-mCherry)pu45. (J and K) Representative images and (K) quantifications of neutrophil recruitment to the tail fin wound. Scale bar, 300 μm. n > 20 in each group. Data representative of three independent experiments. (L and M) Representative tracks and (M) speed of neutrophils’ spontaneous migration in the head mesenchyme in transgenic lines with neutrophil-specific Kir7.1 Q153H overexpression; Tg(lyzC:mCherry)pu35-expressing mCherry alone is used as a control. Scale bar: 50 μm. n > 15 neutrophils from 3 fish of each group. (N and O) Representative images and (O) quantification of the number of neutrophils recruited to the ventral fin upon LTB4 treatment in the indicated zebrafish transgenic lines overexpressing mCherry or Kir7.1Q153H. Scale bar: 50 μm. n > 20 in each group. Data representative of three independent experiments. (B, D, F–H, M, and O) Results are presented as mean ± SD, Mann–Whitney test. (K) Multiple comparisons and Kruskal–Wallis test.

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