Representative flow cytometry sample gating for the spleen. AF, Alexa Fluor; BV, Brillant Violet; FITC, fluorescein isothiocyanate; FSC-A, forward scatter area; FSC-H, forward scatter height; PerCP-Cy5.5, peridinin chlorophyll protein Cy5.5; SB, SuperBright; SSC-A, side scatter area.