Total urine protein in pooled urine samples over time and kidney weight at the end of the study. (A–C) Percent change in total urine protein from baseline values was assessed at day 21 (A) and day 49 (B) of the study. Individual and mean kidney weight (C) at the end of the study. As a minimum of 150 μl of urine was required for total protein analysis, urine samples were pooled in A and B. Pooled samples were extracted from one to four mice per experimental group, and protein analysis was completed in two to three replicates. One kidney (right side) from each mouse was used to determine weight (C). Error bars are ± SEM. Data in C were analyzed using a one-way ANOVA with Tukey correction (P < 0.05).