Changes in frequency of select immune subsets at the end of the study. (A–G) Individual and mean CD4⁺ (A) and CD8⁺ (B) T cell frequency in the spleen. Individual and mean CD8⁻/CD4⁻ DNT (C), CD4⁺ T cell (D), CD8⁺ T cell (E), and CD19⁺ B cell (F) frequencies in the lymph nodes. Individual and mean CD8⁺ T cell frequency in the blood (G). n = 8 per experimental group. Error bars are ± SEM. Data were analyzed using a one-way ANOVA with Tukey correction. *P < 0.05; **P < 0.01; ***P < 0.001.