Changes in immune subsets at the end of the study. (A–D) Individual and mean absolute B and T cell counts in the spleen (A), lymph nodes (B), blood (C), and bone marrow (D). Half of the spleen (A) and three right-hand side lymph nodes (B) were processed for flow cytometry. n = 8 per experimental group. Error bars are ± SEM. Data were analyzed using a two-way ANOVA with Tukey correction. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.