Frequency of live cells at the end of the study. (A–D) Frequency of live cells in the spleen (A), lymph nodes (B), blood (C), and bone marrow (D). Half of the spleen and three lymph nodes from the right side of each mouse were used for detection of live cells in A and B, respectively. n = 8 per experimental group. Error bars are ± SEM. Data were analyzed using a one-way ANOVA with Tukey correction (P < 0.05).