Body weight change and organ weight at the end of the study. (A–C) Individual and mean percent change in body weight from start weight (A). Individual and mean weight of spleen (B) and lymph nodes (C) following necropsy. Six lymph nodes (i.e., two inguinal, two axillary, and two brachial) from each mouse were used to determine weight (C). n = 8 per experimental group. Error bars are ± SEM. Data were analyzed using a one-way ANOVA with Tukey correction. *P < 0.05; ***P < 0.001; ****P < 0.0001.