Cytokinesis in the REEact1 and control S. pombe strains and tetrad analysis when crossed to the myo2-E1 strain. (Α) Representative montages of time-lapse movies of live cells from the indicated strains in the rlc1-GFP background showing mCherry-Atb2 (magenta) and Rlc1-3GFP (green). Images were captured at 3-min intervals. (Β) Violin plots of the analysis of ring constriction (n = 25 cells each) for node coalescence (data written as n; mean ± SD; REE: 20.52 min ± 2.69; MEE: 16.20 ± 2.12; Wilcox test P = 6.16e-07) (i), dwell time (REE: 5.64 min ± 2.64; MEEE: 8.88 min ± 2.52; Wilcox test P = 0.000153) (ii), ring constriction time (REE: 45.48 min ± 12.27; MEEE: 34.32 min ± 6.66; T test P = 0.00029) (iii), total time (REE: 71.64 min ± 13.54; MEEE: 59.40 min ± 7.94; T test P = 0.00037) (iv) and constriction rate (REE: 0.083 μm/min ± 0.021; MEEE: 0.106 μm/min ± 0.019; T test P = 0.00015) (v). (C) Table of counts of the genotypes of colonies arising out of the tetrad dissection of asci from crosses of myo2-E1 and either REE or MEEE strains. Scale bars in A are 1 μm.