Viability, growth, cell size, and actin distribution in the REEact1 and control S. pombe strains. (A) Spot assay showing the growth of 10-fold serial dilutions of the indicated strains that were spotted on YEA plates and grown at 30°C for 3 days. (B) Growth of the four strains fitted to a linear regression model with 95% confidence intervals and placed on a log₁₀ Y axis scale. The dots show the cell counts. (C) Violin plots of the cell size parameters of (i) Length, (ii) Width, (iii) Volume and (iv) Surface Area for the two indicated strains in the wt background (in total 143 and 122 septated cells from 3 sets were measured for the MEEE and REE strains, respectively. Data is mean ± SD; length (μm): MEEE 13.54 ± 0.98, REE: 11.76 ± 0.90, Wilcox test: P < 6.11e-32; width (μm): MEEE 4.10 ± 0.27, REE: 4.24 ± 0.31, Wilcox test: P = 6.25e-04; volume (μm³): MEEE 161.32 ± 24.98, REE: 146.49 ± 23.35, Wilcox test: P = 2.21e-07; surface area (μm²): MEEE 174.45 ± 18.05, REE: 156.56 ± 16.14, Wilcox test: P = 7.04e-15). (D) Phalloidin stainings of the indicated strains in the wt background. (E) Violin plots of the number of fimbrin-GFP patches (i) and patch area at the brightest slice (ii) (data written as n; mean ± SD; counts: MEEE: 34 cells; 45.24 ± 4.42; REE: 37 cells from 3 different experiments; 37.86 ± 5.08; Wilcox test: P = 1.13e-07; area (μm²): MEEE: 144 patches; 0.24 ± 0.05; REE: 144 patches; 0.22 ± 0.04; Wilcox test: P = 1.29e-05;). Scale bars in D are 1 μm.