Approach used to generate the REEact1 and the control S. pombe strains. (A) Schematic of the approach used to generate the REE and MEEE strains. (B) Sequence of the junction between the act1 promoter and the act1 coding sequence (cds) showing the location of the chosen guide RNA target (red) designed in CHOP–CHOP and the expected Cas9 cut site (arrow). (C) Schematic of the act1 locus. (D) Schematic of the expected ubi4-MEEEact1 incorporation at the native act1 locus, including the HDR template region that was used to generate the strains. (E) Schematic of the expected ubi4-REEact1 incorporation at the native act1 locus, including the HDR template region that was used to generate the strains.