The structure of R-β-actin. (A) Representative cryo-TEM image of human filamentous R-β-actin. Scale bar 20 nm. (B) The 2D class averages were obtained from 50 individual regions selected from the filaments, showing the wide and narrow projections of filaments (indicated with lines). (C) 3D reconstruction of human R-β-actin showing five actin subunits in different colors. (D) The D-loop of R-β-actin shows the fit of the protein backbone in the density. Residues in this region are shown in stick representation. (E) Ribbon diagram of the filamentous R-β-actin ADP-binding site, with ADP and interacting residues shown in stick representation. (F) The N terminus region of R-β-actin from residue Ile-4 is shown. Electron density fit to the residues 1 to 3 is missing, as this region is not resolved.
Figure 1.

The structure of R-β-actin. (A) Representative cryo-TEM image of human filamentous R-β-actin. Scale bar 20 nm. (B) The 2D class averages were obtained from 50 individual regions selected from the filaments, showing the wide and narrow projections of filaments (indicated with lines). (C) 3D reconstruction of human R-β-actin showing five actin subunits in different colors. (D) The D-loop of R-β-actin shows the fit of the protein backbone in the density. Residues in this region are shown in stick representation. (E) Ribbon diagram of the filamentous R-β-actin ADP-binding site, with ADP and interacting residues shown in stick representation. (F) The N terminus region of R-β-actin from residue Ile-4 is shown. Electron density fit to the residues 1 to 3 is missing, as this region is not resolved.

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