Centrosomal AURKA accumulation and its functional implications in the G0/G1 transition. (A) Analysis of AURKA accumulation levels at the centrosome along the time course in Fig. 2 E. Representative cropped images at 18 h after serum re-addition and raincloud plots of centrosomal AURKA levels at each time point are shown. Scale bar, 20 µm. Additional representative images are provided in Fig. S5 A. (B) Analysis of the relative positions of the Golgi and centrosome at 18 h after serum re-addition, following the time course in Fig. 3 A. The centers of mass of the nucleus, Golgi, and centrosome were determined from the DAPI, GM130, and γ-tubulin signals, respectively, and the distances between them were calculated. A representative image (siControl) with the centers of mass of the Golgi and centrosome overlaid on the merged image, along with raincloud plots of the measured distances, is shown. Scale bar, 50 µm. (C) Proposed model illustrating the mechanisms regulating the G0/G1 transition.