Analysis of double gene knockdown and marker validation at the G0/G1 transition. (A) Same analysis as in Fig. 3, D and E, but with the addition of double knockdown of AURKA and JNK2. Full data are available in dataset 7. (B and C) Co-staining analysis of cilia and Golgi. Cilia and Golgi were simultaneously stained with antibodies against two respective marker proteins, following the time course shown in Fig. 3 A (including double knockdown of AURKA and JNK2). Representative cropped images of (B) cilia markers IFT88 and acetylated tubulin (AcTub) and (C) Golgi markers GRASP65 and GM130 are shown. Scale bars: (B) 10 µm and (C) 20 µm. For the Golgi markers, the same morphological features were extracted from images of each marker and compared (see plots at the bottom).
Figure S4.

Analysis of double gene knockdown and marker validation at the G0/G1 transition. (A) Same analysis as in Fig. 3, D and E, but with the addition of double knockdown of AURKA and JNK2. Full data are available in dataset 7. (B and C) Co-staining analysis of cilia and Golgi. Cilia and Golgi were simultaneously stained with antibodies against two respective marker proteins, following the time course shown in Fig. 3 A (including double knockdown of AURKA and JNK2). Representative cropped images of (B) cilia markers IFT88 and acetylated tubulin (AcTub) and (C) Golgi markers GRASP65 and GM130 are shown. Scale bars: (B) 10 µm and (C) 20 µm. For the Golgi markers, the same morphological features were extracted from images of each marker and compared (see plots at the bottom).

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