Representative cell images after serum re-addition and features used in analysis. (A) Representative images of ARPE-19 cells for the indicated experimental conditions and time points. Scale bar, 50 µm. (B and C) Correlation matrices of the features used in cell phenotype profiling in Fig. 2 H and Fig. 3 D, respectively. See Table S1 for detailed feature descriptions. (D) Representative fluorescence images used to monitor cell cycle progression and ciliation. Images of ARPE-19 cells were acquired 18 h after serum re-addition (see Fig. 2 E), stained for EdU (via click reaction), Ki-67 (IF), and acetylated tubulin (AcTub; IF). The analysis results are shown in Fig. 3 B. Scale bar, 50 µm. (E) Representative cropped images of ARPE-19 cells used for cell phenotype profiling, related to those shown in Fig. 3 C. Arrows indicate cilia. AcTub, acetylated tubulin. Scale bar, 20 µm. Schematics illustrate typical Golgi morphology in the siControl group, siAURKA- or SP-treated group, and HeLa cells in late G2 (see Fig. S2 B, DMSO).