Epithelial loss of apoptosis does not disrupt lip development. (A) Immunofluorescence and TUNEL assay indicate the absence of cell death in the epithelium of Bak−/−; Baxf/f; CrectTg/0 embryos (n = 5) versus controls (n = 3) at E11.5. MNP, medial nasal process; LNP, lateral nasal process. Scale bars, 70 μm. (B) In control example shown, surfaces were generated from cCasp3+ and DAPI signal at the fusion site. The percentage of cCasp3+ apoptosis in the fusion junction of Bak−/−; Baxf/f; CrectTg/0 embryos (n = 5) is significantly lower than in control embryos (n = 3). Each dot indicates one sample. Error bars represent the SEM. Data were compared using a two-tailed Mann–Whitney t test. (C)Bak−/−; Baxf/f; CrectTg/0 E17.5 embryonic faces appear indistinguishable from controls. Scale bar, 1 mm.
Figure 4.

Epithelial loss of apoptosis does not disrupt lip development. (A) Immunofluorescence and TUNEL assay indicate the absence of cell death in the epithelium of Bak−/−; Baxf/f; CrectTg/0 embryos (n = 5) versus controls (n = 3) at E11.5. MNP, medial nasal process; LNP, lateral nasal process. Scale bars, 70 μm. (B) In control example shown, surfaces were generated from cCasp3+ and DAPI signal at the fusion site. The percentage of cCasp3+ apoptosis in the fusion junction of Bak−/−; Baxf/f; CrectTg/0 embryos (n = 5) is significantly lower than in control embryos (n = 3). Each dot indicates one sample. Error bars represent the SEM. Data were compared using a two-tailed Mann–Whitney t test. (C)Bak−/−; Baxf/f; CrectTg/0 E17.5 embryonic faces appear indistinguishable from controls. Scale bar, 1 mm.

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