Epithelial loss of p120-catenin results in localized epithelial disruption and decreased apoptosis at the fusion junction. (A) Cleft lip (white arrow) is detected in 7/11 of E12.5 Ctnnd1f/f; CrectTg/0 embryos versus controls. Scale bar, 500 μm. (B and C) Histological sections of control and Ctnnd1f/f; CrectTg/0 embryos at 8–10ts (B) (n = 3 each) and 16–22ts (C) (n = 2 for controls, n = 3 for mutants). Black arrowheads point to disrupted epithelium. Scale bars, 50 μm. (D and E) Quantification of apoptosis in the prefusion (D) and fusion (E) region in whole-mount cCasp3-immunostained control and Ctnnd1f/f; CrectTg/0 embryos (n = 3 each for each stage). Dot colors in graphs represent two sides of a single sample. MNP, medial nasal process; LNP, lateral nasal process. Scale bars, 100 μm. Data were compared using two-tailed Student’s t tests.
Figure 3.

Epithelial loss of p120-catenin results in localized epithelial disruption and decreased apoptosis at the fusion junction. (A) Cleft lip (white arrow) is detected in 7/11 of E12.5 Ctnnd1f/f; CrectTg/0 embryos versus controls. Scale bar, 500 μm. (B and C) Histological sections of control and Ctnnd1f/f; CrectTg/0 embryos at 8–10ts (B) (n = 3 each) and 16–22ts (C) (n = 2 for controls, n = 3 for mutants). Black arrowheads point to disrupted epithelium. Scale bars, 50 μm. (D and E) Quantification of apoptosis in the prefusion (D) and fusion (E) region in whole-mount cCasp3-immunostained control and Ctnnd1f/f; CrectTg/0 embryos (n = 3 each for each stage). Dot colors in graphs represent two sides of a single sample. MNP, medial nasal process; LNP, lateral nasal process. Scale bars, 100 μm. Data were compared using two-tailed Student’s t tests.

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