Live imaging reveals actin dynamics at the fusion site. (A) Schematic depicting lip morphogenesis and imaging approach. Yellow arrows indicate prominences coming together; orange arrows indicate the site of tissue fusion. The green box indicates stages of imaging. (B) Schematic of the tissue included in the explant. The white circle indicates the imaged region. (C) Time-lapse stills of labeled F-actin at 6ts reveal initiation of actin enrichment at what will become the fusion junction (n = 10). (D) Time-lapse stills of labeled F-actin at 10ts reveal expansion of actin to multicellular cables (n = 3). (E) Time-lapse stills of labeled F-actin at 16ts reveal zipping of nasal processes (n = 3). Red arrowhead indicates actin cable. MNP, medial nasal process; LNP, lateral nasal process. Time reads as hh:mm. Scale bars, 50 μm.
Figure 1.

Live imaging reveals actin dynamics at the fusion site. (A) Schematic depicting lip morphogenesis and imaging approach. Yellow arrows indicate prominences coming together; orange arrows indicate the site of tissue fusion. The green box indicates stages of imaging. (B) Schematic of the tissue included in the explant. The white circle indicates the imaged region. (C) Time-lapse stills of labeled F-actin at 6ts reveal initiation of actin enrichment at what will become the fusion junction (n = 10). (D) Time-lapse stills of labeled F-actin at 10ts reveal expansion of actin to multicellular cables (n = 3). (E) Time-lapse stills of labeled F-actin at 16ts reveal zipping of nasal processes (n = 3). Red arrowhead indicates actin cable. MNP, medial nasal process; LNP, lateral nasal process. Time reads as hh:mm. Scale bars, 50 μm.

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