Genetic myeloid-specific labeling of CD206⁺ macrophages in tumors. (A) Pseudotime plots of select Mono/Mac subsets in B16F10 tumors from Mujal et al. (2022). (B and C) (B) Gating on the equivalent subsets in B78chOVA tumors by flow cytometry and (C) CD206 expression in each of these subsets. (D) Schematic representation of the Mrc1^{LSL-Venus-DTR} knock-in construct before (WT) and after (DTR) Cre-mediated recombination by crossing to the Csf1r^Cre allele. (E and F) (E) Flow cytometry plots showing reporter (Venus) and CD206 expression in different immune cells in d18 B78chOVA tumors in WT (red) and DTR (blue) mice with (F) quantification of relative reporter expression (DTR – WT) in the different immune cells, segregated by CD206 expression. Fold change of relative reporter expression between the CD206⁺ populations of the different myeloid subsets are noted. (G) Reporter expression of the monocyte and TAM subsets shown in B. (H and I) (H) Average distribution of Venus reporter expressing cells in a typical B78chOVA tumor at d18 and (I) corresponding distribution segregated by CD206 expression. Bar graphs show mean ± SEM (F, G, and I); data are representative of at least two independent experiments, each with at least three biological replicates; WT levels averaged from two biological replicates in F; *P < 0.05, **P < 0.01 by paired t test and RM ANOVA and post-hoc t tests in G.