SPI1 promotes transcription of Ythdf2 in the context of IR. (A) Volcano plot of differentially expressed genes from upregulated (red) and downregulated (blue) transcription pathways of tumor-infiltrating DCs (WT+IR versus WT+control). (B) Bar chart showing the MACS2 score of top 20 ranked TFs binding to Ythdf2. A higher MACS2 score indicates the higher capability of TF binding to Ythdf2. (C) Integrated volcano plot of MACS2 score and differential expressed TF genes, upregulated (red) and downregulated (blue), for Ythdf2 transcription in DCs (WT+IR versus WT+control). (D) ChIP-qPCR results showing SPI1 binding to the promoter region of Ythdf2 (n = 3). (E)Ythdf2 mRNA expression in Spi1 KD DCs cocultured with control or irradiated tumor cells (n = 3). (F) SPI1 protein level in tumor-infiltrating DCs at different days after IR (20 Gy). Statistical analysis was performed using two-sided unpaired Student’s t test (D and E); **P < 0.01; ***P < 0.001. Data are represented as the mean ± SEM. Source data are available for this figure: SourceData F2.