SPI1 promotes transcription of Ythdf2 in the context of IR. (A) GO enrichment analysis of upregulated transcription pathways in tumor-infiltrating DCs of IR versus non-IR. (B) GO enrichment analysis of downregulated transcription pathways in tumor-infiltrating DCs of IR versus non-IR. (C) ChIP-qPCR results showing RELA binding to the promoter region of Ythdf2 (n = 3). (D) ChIP-qPCR results showing BATF binding to the promoter region of Ythdf2 (n = 3). (E–G) ChIP-qPCR results showing SPI1 (E), BATF (F), and RELA (G) binding to the promoter region of Ythdf2 at different time points (n = 3). (H)Ythdf2 mRNA expression in RelA KD DCs with or without irradiation (n = 3). (I)Ythdf2 mRNA expression in Batf KD DCs with or without irradiation (n = 3). (J) mRNA expression of relevant genes in DCs with or without siRNA treatment (n = 3). (K) Quantification of the SPI1 level in Fig. 2 F. Statistical analysis was performed using two-sided unpaired Student’s t test (C, D, and H–J); ns, P > 0.05; ***P < 0.001. Data are represented as the mean ± SEM.