Effect of BNI5 deletion on cell growth, morphology, and Myo1 constriction at 25°C and 37°C. Related to Fig. 8. (A) Growth of bni5Δ cells on YPD plates. Spot assay results were documented after 2 days of incubation at 25°C or 37°C. Strains used: YEF473A (WT) and YEF9654 (bni5Δ). (B) Cell morphology of bni5Δ cells. Cells were cultured in YM-1 at 25°C or 37°C for 24 h prior to imaging. Strains used are the same as in A. (C) Constriction of Myo1 ring during cytokinesis in WT and bni5Δ cells. The temporal changes in the diameter of the Myo1 ring were quantified from time-lapse series taken with 1-min intervals. n denotes the number of cells analyzed per strain. Bold lines and shaded bands represent the mean and SD, respectively. P values were determined using a two-sided unpaired t test. Strains used: YEF11385 (MYO1-GFP mScarlet-TUB1) and YEF11443 (bni5Δ MYO1-GFP mScarlet-TUB1).
Figure S5.

Effect of BNI5 deletion on cell growth, morphology, and Myo1 constriction at 25°C and 37°C. Related to Fig. 8. (A) Growth of bni5Δ cells on YPD plates. Spot assay results were documented after 2 days of incubation at 25°C or 37°C. Strains used: YEF473A (WT) and YEF9654 (bni5Δ). (B) Cell morphology of bni5Δ cells. Cells were cultured in YM-1 at 25°C or 37°C for 24 h prior to imaging. Strains used are the same as in A. (C) Constriction of Myo1 ring during cytokinesis in WT and bni5Δ cells. The temporal changes in the diameter of the Myo1 ring were quantified from time-lapse series taken with 1-min intervals. n denotes the number of cells analyzed per strain. Bold lines and shaded bands represent the mean and SD, respectively. P values were determined using a two-sided unpaired t test. Strains used: YEF11385 (MYO1-GFP mScarlet-TUB1) and YEF11443 (bni5Δ MYO1-GFP mScarlet-TUB1).

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